Evaluation of cell mortality with acridine orange/ethidium bromide (AO/EB) staining after exposition to chemotherapeutic agents.
- Manolo D’Arcangelo (2211607)
- Matilde Todaro (297592)
- Jessica Salvini (2211613)
- Antonina Benfante (731279)
- Maria Luisa Colorito (731280)
- Armida D’Incecco (2211616)
- Lorenza Landi (2211604)
- Tiziana Apuzzo (164320)
- Elisa Rossi (730558)
- Spartaco Sani (2211610)
- Giorgio Stassi (297602)
- Federico Cappuzzo (2211619)
Publication date
May 2015
DOIAbstract
<p>AO is a vital dye and stains both live and dead cells. EB penetrates into cells with disrupted cytoplasmic membrane, staining only dead cells. Therefore, viable cells appear uniformly green and dead cells are labelled in orange. a) Low sensitivity (cell mortality 5%). b) Average sensitivity (cell mortality 40%). c) High sensitivity (cell mortality 80%).</p
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