Genistein effect on <i>i</i>Ca²⁺ response to NMDA and ATP response.

<p>(<b>A</b>) ΔF/F₀ averaged response in Fluo-4-AM-labeled rCCA perfused with 1 mM NMDA in the presence of genistein (GX). (<b>B</b>) ΔF/F₀ averaged response in Fluo-4-AM-labeled rCCA perfused with ATP. Line above traces indicates perfusion with 1 mM NMDA after 120 sec basal recording. Line below (if applicable) indicates the inhibitor used throughout the recording time. (<b>C</b>) ∫(ΔF/F₀) distribution histogram for cell population responses in genistein and ATP conditions; the NMDA treated population distribution is included for comparison. The RTV value is indicated by the black arrowhead (see text). Statistical analysis for these distributions with the number of cells and the number of experiments are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126314#pone.0126314.t001" target="_blank">Table 1</a>. Representative images from <i>i</i>Ca²⁺ responses are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126314#pone.0126314.s008" target="_blank">S8 Fig</a>.