Activation of <i>dsx-GAL4</i>-expressing neurons in females induces oviposition-type extension of the ovipositor and egg-laying.

<p>(<b>A</b>) Extrusion of the ovipositor in response to male courtship in mated wild-type females. (<b>B</b>) Egg-laying in mated wild-type females. (<b>C, C’</b>) The oviposition posture with ovipositor extension (<b>C</b>) and egg-laying (<b>C’</b>) was artificially induced in mated females that express dTrpA1 under the control of <i>dsx</i>^<i>GAL4</i><i>(G)</i> by a temperature increase to 29°C. (<b>D</b>) The proportion of mated (left-hand graph) and virgin (right-hand graph) flies that engaged in the ovipositor extension or egg-laying upon temperature increases up to 32°C was compared among the 4 genotypes indicated at the bottom. (+) and (–) indicate the presence or absence of <i>dsx</i>^<i>GAL4</i><i>(G)</i> and <i>dTrpA1</i> in the fly groups examined. (<b>E</b>) Cumulative plots of the number of <i>dsx</i>^<i>GAL4</i><i>(G)>dTrpA1</i> mated females (n = 40) that exhibited ovipositor extension or egg-laying when the ambient temperature was increased from 22°C to 32°C. (<b>F</b>) The proportion of virgin flies (<i>dsx</i>^<i>GAL4</i><i>(G)>dTrpA1</i>) exhibiting ovipositor extension or egg-laying upon a temperature increase to 32°C was compared at different ages: 0 (within 24 h), 3, 5, and 8 days after eclosion. The numbers shown in parentheses (D, F) indicate the number of flies examined.