Behavioral MARCM identifies the neurons that initiate mating-type ovipositor extrusion or oviposition-type extension/egg ejection.

<p>(<b>A-D</b>) Oviposition posture and egg-laying (<b>A</b>) and mating-type extrusion (<b>C</b>) observed in flies carrying MARCM clones that express dTrpA1 in <i>dsx-GAL4</i>-positive cells. (<b>B, D</b>) Labeling pattern of GFP expression in MARCM females. Each vertical column represents scores for a single fly. Neuron classes indicated in the left-side column are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.g003" target="_blank">Fig 3A</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.s001" target="_blank">S1 Fig</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.s004" target="_blank">S1 Table</a>. * in <b>B</b> indicates that the images shown in Fig 4A, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.s003" target="_blank">S3A</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.s003" target="_blank">S3B</a> Fig, and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.s014" target="_blank">S8 Movie</a> are for this fly, and # in <b>D</b> indicates that the images shown in Fig 4C, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.s003" target="_blank">S3C</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.s003" target="_blank">S3D</a> Fig, and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126445#pone.0126445.s015" target="_blank">S9 Movie</a> are for this fly. (<b>E</b>) The proportion of flies that carried mCD8::GFP-labeled dTrpA1-expressing cells in the indicated cluster is compared between the non-responder group (Responder-N, n = 84) and responder group (Responder-MO, n = 67). The responder group included flies that exhibited either oviposition-type extension/egg-laying or mating-type extrusion. (<b>F)</b> The proportion of flies that carried mCD8::GFP-labeled dTrpA1-expressing cells in the indicated cluster is compared between the fly group that responded with oviposition-type extension/egg-laying (Responder-O, n = 38) and the fly group that did not show oviposition-type extension/egg laying (Responder-NM, n = 113). (<b>G</b>) The proportion of flies that carried mCD8::GFP-labeled dTrpA1-expressing cells in the indicated cluster is compared between the fly group that responded with the mating-type extrusion (Responder-M, n = 29) and the fly group that did not show mating-type extrusion (Responder-NO, n = 122). * p < 0.05 and **** p < 0.0001, by Fisher’s exact probability test.