Different types of recombineering processes.

<p>(A) Insertional recombination. A selection marker (sm) containing homology to a target site is inserted during the process of DNA replication (dashed line). Gap repair cloning. A gapped plasmid with terminal homology regions to a target site is used to subclone a sequence of interest. (C) Subcloning plus insertion (SPI). Insertion of a cassette occurs simultaneously during subcloning and generates a targeted subcloned plasmid. The template DNA remains unmodified.