<p>(A) RT-PCR analysis of Hh target genes in several cancer cell lines treated with 500 ng/ml recombinant Shh for 48 h. (B) Quantitative real-time PCR analyses of Hh target genes expression in A. Error bars represent the mean ± SD of five independent experiments. <b>*</b><i>P</i> < 0.05, <b>**</b><i>P</i> < 0.01. (C) The cancer cell lines were cultured for 5 days after treatment with 500 ng/ml recombinant Shh and analyzed by WST-8 assays at day 5. Error bars represent the mean ± SD of five independent experiments. <b>*</b><i>P</i> < 0.05.</p
<p>Waterfall plot representing LogP RSA scores for sgRNAs targeting A) SMYD2 and B) SMYD3. 313 cell ...
<p>The expression level of selected genes was examined using real-time RT-PCR in a normal and a canc...
<p><b>(A)</b> Three different human RCC derived cell lines were seeded on 96-well plates at a concen...
<p>(A) Quantification of SHH expression by Q-RT-PCR in a panel of 45 human colorectal tumors and pai...
<p>A. Real-time qRT-PCR for the expression levels of CDO and Hh signaling components (SHH, PTCH1 and...
<p>U251MG cells were knocked down by siRNAs, followed by treatment with conditioned medium collected...
<p>(A) <i>In vitro</i> cytotoxicity assay showing MTT activity across 9 days of induction in differe...
<p><b>A:</b> Cell proliferation assay by CCK-8 kit corresponding to the treatments in (<a href="http...
<p>Error bars represent standard error of the fold change. SHH was expressed 168-fold higher in untr...
<p>The relative effect of two independent shRNAs per kinase on cell growth in LHS (A) and MCF10A (B)...
<p>(A) Proliferation, as measured by [<sup>3</sup>H]-thymidine incorporation, of the indicated cell ...
<p>(A) Western blotting of BBF2H7 in U251MG cells knocked down by siRNAs. Scramble and siBBF2H7 indi...
<p>(A) qRT-PCR verification of <i>hPNPase<sup>old-35</sup></i>-putative “<i>directly</i>” regulated ...
<p>A. Total cell lysates of human renal carcinoma 786-O cells stably expressing the control shRNA (S...
<p>(A) Mock-AGS and H4R-AGS cells were treated with 10<sup>−5</sup> M histamine, CB or CB accompanie...
<p>Waterfall plot representing LogP RSA scores for sgRNAs targeting A) SMYD2 and B) SMYD3. 313 cell ...
<p>The expression level of selected genes was examined using real-time RT-PCR in a normal and a canc...
<p><b>(A)</b> Three different human RCC derived cell lines were seeded on 96-well plates at a concen...
<p>(A) Quantification of SHH expression by Q-RT-PCR in a panel of 45 human colorectal tumors and pai...
<p>A. Real-time qRT-PCR for the expression levels of CDO and Hh signaling components (SHH, PTCH1 and...
<p>U251MG cells were knocked down by siRNAs, followed by treatment with conditioned medium collected...
<p>(A) <i>In vitro</i> cytotoxicity assay showing MTT activity across 9 days of induction in differe...
<p><b>A:</b> Cell proliferation assay by CCK-8 kit corresponding to the treatments in (<a href="http...
<p>Error bars represent standard error of the fold change. SHH was expressed 168-fold higher in untr...
<p>The relative effect of two independent shRNAs per kinase on cell growth in LHS (A) and MCF10A (B)...
<p>(A) Proliferation, as measured by [<sup>3</sup>H]-thymidine incorporation, of the indicated cell ...
<p>(A) Western blotting of BBF2H7 in U251MG cells knocked down by siRNAs. Scramble and siBBF2H7 indi...
<p>(A) qRT-PCR verification of <i>hPNPase<sup>old-35</sup></i>-putative “<i>directly</i>” regulated ...
<p>A. Total cell lysates of human renal carcinoma 786-O cells stably expressing the control shRNA (S...
<p>(A) Mock-AGS and H4R-AGS cells were treated with 10<sup>−5</sup> M histamine, CB or CB accompanie...
<p>Waterfall plot representing LogP RSA scores for sgRNAs targeting A) SMYD2 and B) SMYD3. 313 cell ...
<p>The expression level of selected genes was examined using real-time RT-PCR in a normal and a canc...
<p><b>(A)</b> Three different human RCC derived cell lines were seeded on 96-well plates at a concen...