Immunohistochemical detection of myeloperoxidase.

<p><b>(A)</b> Detection of myeloperoxidase (MPO) by immunohistochemistry in pancreatic tissue sections four, eight and 24 hours after induction of acute pancreatitis in wild-type C57BL/6 and <i>Bace1</i>^<i>-/-</i> mice. Representative images are shown in 200x magnification. A polyclonal rabbit antibody (anti-MPO, Abcam) was used in an optimized dilution of 1:200 to keep unspecific signals within the parenchyma to a minimum. Staining was visualized using DAB. Arrows indicate MPO positive inflammatory cells. Scale bar represents 50 μm in all images. <b>(B)</b> MPO positive cell counts per field (200x) are shown for wild type and <i>Bace1</i>^<i>-/-</i> mice during the course of disease. Symbols represent individual mice. Line represents mean. n.s. = not significant.