Effect of enoxaparin fractions on interleukin release.

<p>Ion-exchange chromatographic (IC) separation of enoxaparin. The separations were performed using a CarboPac PA100 semi-preparative column; optimised NaCl gradient was from 32–74% over 70 minutes with a flow rate of 2 mL/minute and UV detection wavelength of 232 nm. The numbers indicate the area of all the fractions collected. Data represents a typical experiment out of ten independent experiments <b>(A)</b>. Inhibition of IL-6 <b>(B)</b> and IL-8 <b>(C)</b> release by IC-derived 14 fractions of enoxaparin (60 μg/mL) after trypsin-induced <i>in-vitro</i> stimulation of epithelial cells. The relative percentile amount of each fraction (fraction 1 to 14) present in 60 μg/mL of intact enoxaparin was: 9.18%, 4.24%, 6.12%, 5.15%, 2.99%, 4.78%, 20.32%, 11.4%, 10.53%, 7.35%, 5.98%, 4.6%, 2.95% and 5.06% respectively. Data is presented as percentage (mean ± SD) of the maximal observed IL-6 and IL-8 concentrations. *<i>p</i><0.05, and ***<i>p</i><0.001 versus trypsin-stimulated control.