Effect of SILD on cardiac hypertrophy and macrophage infiltration.

<p>(A) Representative photomicrographs of H&E-stained heart from CTRL, SILD, STZ and STZ+SILD mice on harvesting (bar represents 100μm).(B) Histogram shows heart cross-sectional area. Results are expressed as mean ± SD (n = 4 in each group). ^*P <0.05 STZ vs CTRL; ^#P <0.05 STZ+SILD vs STZ (Student’s t-test).(C) Histogram shows mRNA quantitative analysis ANP. Results are expressed as mean ± SD (n = 3 in each group). ^*P <0.05 STZ vs CTRL; ^##P <0.01 STZ+SILD vs STZ (Student’s t-test).(D) β-MHC and GAPDH expression detected by Western blotting. Histogram shows activation of hypertrophic protein in STZ mice that was reduced in STZ+SILD mice. Results are expressed as mean ± SD (n = 3 in each group) ^##P <0.01 STZ+SILD vs STZ (Student’s t-test).(E) Histogram illustrates percentage of infiltrating F4/80⁺ cells in the heart from CTRL, SILD, STZ and STZ+SILD mice. Results are expressed as mean ± SD (n = 4 in each group). ^*P <0.05 STZ vs CTRL; ^#P <0.0 STZ+SILD vs STZ (Student’s t-test).(F) Histograms indicates percentage of F4/80⁺TIE2⁺ cells. Expression of F4/80⁺TIE2⁺ cells was decreased in STZ compared with CTRL mice and increased in STZ+SILD compared with STZ mice. Results are expressed as mean ± SD (n = 4 in each group), ^*P <0.05 STZ vs CTRL; ^##P <0.01 STZ+SILD vs STZ (Student’s t-test).(G) iNOS and COX-2 expression detected by Western blotting. Histogram shows induction of inflammatory proteins in STZ mice that was reduced in STZ+SILD mice. Results are expressed as mean ± SD (n = 3 in each group). ^**P <0.01 STZ vs CTRL, ^##P <0.01 STZ+SILD vs STZ (Student’s t-test).