<p>WT, wild type parental strain; KO, <i>Bbtpx-1</i> KO parasite; GI, <i>Bbtpx-1</i> genome integrant; and E, <i>Bbtpx-1</i> episomal transfectant. (A) Regions for <i>Bbtpx-1</i> (Bbtpx-1 5’ F×Bbtpx-1 R), hDHFR expression cassette (dhfr F×Bbtpx-1 3’ R), and RFP-BSD expression cassette (Bsd F×Bbtpx-1 3’ R) were PCR-amplified with specific sets of primers from parasites' gDNA. M, 1 kb DNA ladder marker. (B) Evaluation of a cloned genome integrant, which showed both GFP and RFP fluorescence. The proposed structure of the <i>Bbtpx-1</i> gene locus based on the diagnostic PCR assay results is shown in the left diagram. (C) Southern blot analysis. <i>Bbtpx-1</i> 5' UTR probe is indicated. Arrows in the schematic diagram indicate the expected loca...
<p>KO, <i>Bbtpx-1</i> KO parasites were previously derived (Asada et al., 2012). GI, cloned <i>Bbtpx...
(A) Schematic of the endogenous C-terminal tagging with mCherry. Constructs were integrated into par...
<p>A) Schematic representation of the plasmid pL0017.1.2-5’FR-PbPL-V5 used to transfect marker-free ...
<p>Expression of <i>Bbtpx-1</i> in the wild type parental strain (WT), <i>Bbtpx-1</i> KO parasites (...
<p><b>A</b>. Schematic of the proposed integration site of <i>pEf-msa-1-Bm86ep-gfp-bsd</i> into the ...
<p>(<b>A</b>) Schematic representation of (i) the PgCS/Pb<sup>RR</sup> targeting construct, (ii) the...
<p>(A) Diagrammatic representation of the <i>pbggcs</i> replacement vectors (top), the <i>pbggcs</i>...
<p><i>A</i>. Schematic representation of endogenous <i>PfLplA1</i> gene locus (I.), transfection pla...
<p>Organizational maps of plasmid PyrFlu (A), the construction strategy of recombinant vector PyrFlu...
<p>A) Schematic diagram of the endogenous <i>pbpkg</i> locus, the targeting construct and the transg...
<div><p>(A) Schematic diagram showing integration of the construct pV<sub>b</sub>BB/IDH into the <i>...
<p>(A) Integration strategy to generate <i>Pb</i>red parasites. The <i>PbHSP70/1</i> genomic locus w...
<p><b>A.</b> Diagram of the <i>pbggcs</i> overexpression plasmid (Top), the <i>dssurrna</i> genomic ...
<p>(A) Southern analysis on digested gDNA from unselected and WR-selected 3D7/pBK<sub>min</sub>C par...
<p>Schematic drawing shows the three genomic loci at <i>α-tubulin II</i>, <i>calmodulin</i>, and <i>...
<p>KO, <i>Bbtpx-1</i> KO parasites were previously derived (Asada et al., 2012). GI, cloned <i>Bbtpx...
(A) Schematic of the endogenous C-terminal tagging with mCherry. Constructs were integrated into par...
<p>A) Schematic representation of the plasmid pL0017.1.2-5’FR-PbPL-V5 used to transfect marker-free ...
<p>Expression of <i>Bbtpx-1</i> in the wild type parental strain (WT), <i>Bbtpx-1</i> KO parasites (...
<p><b>A</b>. Schematic of the proposed integration site of <i>pEf-msa-1-Bm86ep-gfp-bsd</i> into the ...
<p>(<b>A</b>) Schematic representation of (i) the PgCS/Pb<sup>RR</sup> targeting construct, (ii) the...
<p>(A) Diagrammatic representation of the <i>pbggcs</i> replacement vectors (top), the <i>pbggcs</i>...
<p><i>A</i>. Schematic representation of endogenous <i>PfLplA1</i> gene locus (I.), transfection pla...
<p>Organizational maps of plasmid PyrFlu (A), the construction strategy of recombinant vector PyrFlu...
<p>A) Schematic diagram of the endogenous <i>pbpkg</i> locus, the targeting construct and the transg...
<div><p>(A) Schematic diagram showing integration of the construct pV<sub>b</sub>BB/IDH into the <i>...
<p>(A) Integration strategy to generate <i>Pb</i>red parasites. The <i>PbHSP70/1</i> genomic locus w...
<p><b>A.</b> Diagram of the <i>pbggcs</i> overexpression plasmid (Top), the <i>dssurrna</i> genomic ...
<p>(A) Southern analysis on digested gDNA from unselected and WR-selected 3D7/pBK<sub>min</sub>C par...
<p>Schematic drawing shows the three genomic loci at <i>α-tubulin II</i>, <i>calmodulin</i>, and <i>...
<p>KO, <i>Bbtpx-1</i> KO parasites were previously derived (Asada et al., 2012). GI, cloned <i>Bbtpx...
(A) Schematic of the endogenous C-terminal tagging with mCherry. Constructs were integrated into par...
<p>A) Schematic representation of the plasmid pL0017.1.2-5’FR-PbPL-V5 used to transfect marker-free ...