Regulation of IL-6 signaling pathway molecules by curcumin.

<p>HuF cells were treated with either vehicle (control), IL-1β (10ng/ml), or with a combination of IL-1β (10ng/ml) and curcumin (30μM), or curcumin alone (30μM). Total mRNA was analyzed for IL-6 (A), IL-6R (C) and gp130 (D) expression by qPCR. (B) Expression of IL-6 at protein level was examined by Western blot analysis. The values are expressed as the means ± S.E. (<i>n</i> = 3). ***, <i>p</i> < 0.001.