Effect of DENV-2 infection on PML-NBs distribution, number and size.

<p>DENV-2 infected A549 cells were fixed 24 h p.i. and a double immunofluorescence staining was performed. (A) Viral antigen expression was visualized by using monoclonal anti-E antibody and FITC-labelled anti-mouse IgG2a. PML was visualized by using anti-PML polyclonal antibodies and TRITC-labelled anti-rabbit IgG1. Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI). Cells were visualized by confocal microscopy. Magnification: 600 X. (B) Quantification of the mean number of PML-NBs in the nuclei of non-infected, DENV-2 infected and DENV-2 infected neighboring A549 cells at 24 h p.i. 50 cell nuclei were counted for each condition. The reported values are mean ± SD. (C) Determination of the mean diameter of PML-NBs in non-infected, DENV-2 infected and DENV-2 infected neighboring A549 cells represented as a box and whiskers plot. The box extends from the 25th percentile to the 75th percentile. The horizontal line represents the median and the whiskers extend down to the smallest value and up to the largest. PML-NB sizes from 100 non-infected, DENV-2 infected or DENV-2 infected neighboring cells were measured. Asterisks indicate a significant difference (*** p < 0.001; ** p < 0.01; * p < 0.05).