SP-A and SP-R210_L modulate responsiveness of macrophages to LPS.

<p>Control and SP-R210_L(DN) cells were pretreated with SP-A purified by either method 1 (SP-Am1) or method 2 (SP-Am2) as described in “Materials and Methods”. Cells were pretreated with indicated amounts of SP-Am1 (A) or SP-Am2 (B) for 24 hrs and then incubated with 100 ng/mL LPS. Levels of secreted TNFα were measured in media by ELISA at 4 hrs after addition of LPS. Data shown are means±SD, n = 3 representative of 3–6 independent experiments. Lines indicate significant differences between indicated groups at *p <0.0001; ^#p<0.04; ^$p<0.005