Size-exclusion chromatography of the wild type HspB1 (A) and its G34R (B), P39L (C), and E41K (D) mutants performed in buffer S (20 mM Tris/acetate pH 7.6, containing 150 mM NaCl, 0.1 mM EDTA, 0.1 mM PMSF and 15 mM ME) on a Superdex 200 column at room temperature.

<p>150 μl of sample containing variable quantities of protein (10–120 μg) were loaded on the column and eluted at a flow rate of 0.5 ml/min. Normalized elution profiles obtained after loading on the column 10 (dotted lines), 30 (dash-dotted lines), 60 (dashed lines) and 120 (solid lines) μg of protein are presented.