Effect of acute AICAR administration on mitochondrial FAT/CD36 content.

<p>An intraperitoneal injection of AICAR decreased blood glucose (A) and increased skeletal muscle AMPK Thr172 phosphorylation without altering total AMPK protein content (B). The FAT/CD36 antibody in mice is polyclonal, and therefore we confirmed that an ~88 kDa band was present in giant sarcolemmal vesicle (GSV) and mitochondrial samples from FAT/CD36 WT mice and absent in mitochondria isolated from FAT/CD36 knock out (KO) mice. A representative blot is provided (C). AICAR increased FAT/CD36 protein content in subsarcolemmal (SS) but not in intermyofibrillar (IMF) mitochondria. COXIV was utilized as a loading control. Muscle was taken 1 hour after the intraperitoneal injection of AICAR. Values are reported as the means ± SEM (n = 6). * significantly (P<0.05) different from saline.