Anti-SW731 effect of κ-carrageenan on MDCK cells.

<p>(A, B) MDCK cells were incubated with SW731, CA04, PR8, WSN, and ZB07 at 4°C (MOI = 0.1) and then treated with κ-carrageenan or Ribavirin at the indicated concentration after removal of the virus inoculum. After 24 h, the viral titers were determined by HA and TCID50 assays. (C, D) MDCK cells were incubated with SW731, CA04, PR8, WSN, and ZB07 at 4°C (MOI = 1) and then treated with κ-carrageenan or Ribavirin at the indicated concentration after removal of the virus inoculum. After 24 h, the viral titers were determined by HA and TCID₅₀ assays. (E) MDCK cells were infected with SW731 at an MOI of 1 and then treated with κ-carrageenan at the indicated concentration after removal of the viral inoculum. After 48 h, CPE inhibition was determined by CPE assay. Results were analyzed with the independent sample t test (n = 3). Values are means ± SEM. Significance: *<i>P <</i> 0.05 vs. nondrug treated control group; **<i>P <</i> 0.005 vs. nondrug treated control group. Results are representative of two independent experiments.