Tra2β Alteration in HIV-1 Splice Site Usage is Independent of ESE3 and ESS3.

<p>A, Schematic of the HIV-1 provirus and which viral sequences were used in the generation of the SV-1 env expression vector. Shown is SV40 promoter (SV40), the polyadenylation signal (pA), the position of the splice donors (SD) and splice acceptors (SA) as well as the location of ESE3 and ESS3. SV-1 envΔES was generated by deletion of ESE3 and ESS3. B, 293T cells were transfected with SV-1 env or SV-1 envΔES along with CMVmyc (mock), CMVmyc Tra2β (Tra2β) or CMVmyc Tra2βΔN (Tra2βΔN). RNA was extracted 48h post-transfection and RT-PCR assays performed for HIV-1 MS RNAs as detailed in “Material and Methods”. Shown on the left are representative gels of the RT-PCR products detected and on the right, a summary of the results obtained from n = 3 assays, indicating the relative abundance of a subset of the RT-PCR products detected; U designates unspliced RNA. Asterisks indicated values deemed significant from control (mock) at p<0.05.