Changes in auxin homoeostasis lead to altered cell specification.

<p><b>A, D,</b> WT. <b>B and C,</b> <i>yuc1/yuc1 yuc2/YUC2</i>. <b>E,</b> <i>yuc8/yuc8</i>.<b>A-C,</b> green signal indicates the expression of central cell specific marker, and red indicates egg cell specific marker. The yellow color observed results from the overlapping of the red colour (egg or egg-like nuclei) on the green background of the central cell in the confocal image. <b>D-E,</b> green signal indicates synergid cell-specific marker, and red indicates egg cell-specific marker. <b>A,</b> WT ovule showing a single egg cell (Red) and a central cell (green) at FG7 stage. <b>B,</b> A mutant ovule at FG7 stage showing 2 egg cells and 1 central cell. <b>C,</b> an FG7 mutant ovule showing 3 egg cells. <b>D,</b> A WT ovule showing normal polarization of synergid nuclei towards the micropylar end. <b>E,</b> merged image of a <i>yuc8</i> mutant ovule, showing miss-positioned synergids. The white arrows indicate the egg cell marker expression. Scale bar, 20 μM.