Effects of PPAR-β/-γ antagonists on nifedipine-mediated ROS production.

<p>Platelets were preincubated with nifedipine or nifedipine combined with GSK0660 or GW9662 for 3 min; then NBT or DCFH-DA was added, followed by collagen to measure superoxide (A) and hydrogen peroxide (B) formation respectively. Data were expressed as means ± SEM (<i>n</i> = 5). ^***<i>p</i> < 0.001 compared with collagen alone group; ⁺⁺<i>p</i> < 0.01 compared with respective collagen+nifedipine treated group.