PKA and Sch9 regulate pol III transcription through additional effectors other than Maf1.

<p>Northern analysis of pol III transcription in analog-sensitive strains. <i>pka-as</i>, <i>sch9-as</i> and <i>pka-as sch9-as</i> strains (abbreviated to <i>4-as</i> in panel B) were treated with 1NM-PP1 or drug vehicle for 1 hour. <b>A.</b> Pol III transcription reported by the amount of pre-tRNA^Leu transcript is normalized to U3 snRNA, expressed relative to the untreated wild-type strain and indicated below each lane. Transcription is sensitive to loss of either PKA or Sch9 catalytic activity and the reduction is Maf1-dependent (right-most 2 lanes). Transcription for <i>maf1</i>Δ <i>pka-as sch9-as</i> normalized to its <i>maf1</i>Δ control. <b>B.</b> Pol III transcription is sensitive to loss of PKA and Sch9 activity in a strain that contains alanine-substituted Maf1 (<i>MAF1-7SA</i>). An ATP analog-insensitive <i>maf1</i>Δ::natR strain and a <i>pka-as sch9-as maf1</i>Δ::natR strain, each containing a plasmid with <i>MAF1</i> wild-type (WT) or 7SA alleles were assayed and quantified as in panel A using U4 snRNA for normalization. The framed regions in panels A and B represent non-adjacent lanes from the same gels.