Immunohistochemical detection of Der p 1, IgE and CD68, and the presence of eosinophil leukocytes in nasal biopsies before and after <i>in vivo</i> challenge with Der p 1 allergen.

<p>H-E staining for Eosinophils; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127477#sec006" target="_blank">Methods</a>.</p><p>*Double staining with Der p 1.</p><p>** Staining of cells and the mucous in the epithelium, lamina propria and the submucosal glands. An ordinal scale was used for intensity of Der p 1 staining. This allows for rank order (negative, 1+, 2+, 3+, 4+), but does not allow for measuring relative degree of difference between them.</p><p>***Comparison between non-challenged (pre) and challenged (post) tissue.</p><p>****Comparison between atopic and nonatopic (non-challenged tissue).</p><p>EP: nasal mucosal epithelium; LP: lamina propria; GL: subepithelial mucous glands.</p><p>Number of cells: Mean (range); NA: not available; Statistics: t-test was used for comparison between means.</p><p>Immunohistochemical detection of Der p 1, IgE and CD68, and the presence of eosinophil leukocytes in nasal biopsies before and after <i>in vivo</i> challenge with Der p 1 allergen.