Cytotoxicity profile of six iron compounds on HK-2 cells.

<p>The cells were exposed to the different iron compounds in doses ranging from 0.1 to 0.8 mg/ml for 24 hours. During the final 4 hours, MTT was added (top figure, A). The MTT assay was carried out to establish cell viability. In a separate set of experiments from those used for the MTT assays, cells were exposed to the different iron compounds at the ranges indicated for 24 hours. In a separate set of experiments, plasma membrane integrity was evaluated by LDH assay. The amount of lactate dehydrogenase (LDH) released from the cells was compared to the control group (bottom figure, B).. Data represents the mean±SEM from five independent experiments. *: compared to the control p<0.05.