Characterization of Est10 esterase activity.

<p>(A) Determination of chain length specificity using pNP esters of fatty acids: acetate (C2), butyrate (C4), and decanoate (C10. (B) Effect of the pH on esterase activity of Est10. (C) Thermal stability of Est10. Est10 was pre-incubated for 30 min at temperatures ranging from 30°C to 65°C before determining its residual activity. The control was not pre-incubated. (D) Effect of the temperature on the esterase activity of Est10. The reaction was carried at temperatures ranging from 30°C to 55°C. Except when noted, reactions were performed at 40°C using pNP butyrate as substrate. In all cases averages of triplicate assays are shown and error bars represent standard deviation.