BLS signals B16 cells directly via TLR4.

<p>B16 cells were preincubated <i>in vitro</i> with 100 μg of BLS or 5 ng of LPS. After 48h cells were washed and 2.5x10⁵ cells were sc inoculated in the right flank of C57BL/6J mice (A). To block TLR4, cells were incubated with TLR4/MD2 monoclonal antibody; then they were stimulated with 100 μg of BLS (BLS+TLR4/MD2 Ab) and inoculated into C57BL/10ScNJ (B) and C57Bl/6J (C) mice. Figures show the survival rate; n = 7, * p<0.05, *** p< 0.001.