Circulating pore-forming PLY mediates cardiac injury and inflammation <i>in vivo</i>.

<p>(<b>A</b>) Representative Western blots showing circulating cTnI and cTnT in murine plasma following i.v. injection of PLY (200 ng/g) and PdB (400 ng/g). (<b>B</b>) Mean±SD of circulating cTnI from 4 mice (each group) that survived at 24 h after treatment with PLY and PdB. *<i>p</i><0.05 as compared to PdB group. (<b>C</b>) Survival curves of mice injected (i.v.) with PLY (400 and 200 ng/g) or PdB (400 ng/g) (n = 8 each group). (<b>D</b>) Pathological examination of murine heart sections after infection with purified PLY (200 ng/g) (<i>a</i>, <i>b</i> and <i>d</i>) and PdB (400 ng/g) (<i>c</i>). H&E representative images (<i>a-c</i>) of murine heart sections under x60 magnification showing inflammatory cell infiltrations (arrows) in mice dying within 6 hours (<i>a</i>) and those surviving for 24 hours (<i>b</i>). (<i>c</i>) PdB injection causes no pathological abnormality in murine myocardium. (<i>d</i>) Absence of active caspase-3 staining in murine myocardium following PLY injection.