Chymotrypsin inhibition assays.

<p>(A) Baseline chymotrypsin (0.1 μg/mL) activity with <i>N</i>-benzoyl-L-tyrosine ethyl ester (0.5 mM). Inhibition of chymotrypsin with (B) Roche protease inhibitor cocktail (60 μg/mL), (C) TFP4 (2 μg/mL), and (D) crude protein extract (1.2 μg/mL) from the defense gland secretion of <i>C</i>. <i>formosanus</i>. Additions are indicated by arrows. The hydrolysis rate and correlation coefficient (R²) are shown for each reaction.