Tumor vessel perfusion.

<p>The vascular function is increased in tumors of RT^posSG^ko versus RT^posSG^wt mice. 15 week RT^posSG^wt (a) and RT^posSG^ko (b) mice were perfused with FITC-lectin, which was introduced to the circulation prior to sacrifice of the animals and excision of the pancreas. Tumor sections were stained for the endothelial marker CD31 (red), the localization of which was compared to lectin (green) that had immobilized on the luminal side of endothelial cells in perfused vessels. The ratio between immobilized lectin and CD31 staining was calculated to determine the fraction of perfused, and thus functional vessels (c). Each data point in represents an individual animal. Statistical analysis was performed using a two-tailed Mann-Whitney test. Error bars represent mean ± SEM.