Overexpression of Tpm3.1 alters the F/G actin ratio.

<p>(A) 10 μg of total cellular protein from the B35 clones together with skeletal actin protein (0.025, 0.05, 0.1, 0.25 μg) was analysed by SDS-PAGE followed by western blotting and probe with an actin antibody and GAPDH as loading control. A representative blot is shown from <i>n</i> = 3 independent experiments. (B) Actin levels were determined by densitometry and expressed as ng of actin per μg of total cellular protein, <i>n</i> = 3. (C) Biochemical fractionation of the G and F-actin pools from the different Tpm-overexpressing B35 clones were performed followed by SDS-PAGE electrophoresis. Shown are representative western blots probed for actin. (D) The F-actin/G-actin ratio was determined by densitometric scan of the blots. Shown are the mean ± SEM, <i>n</i> = 5. **<i>P</i><0.01 compared to control.