Impact of the different Tpm isoforms on the organization of the actin cytoskeleton.

<p>(A) Representative immunofluorescence images of the Tpm-overexpressing B35 clones stained with 488-Atto phalloidin to visualise F-actin (green) and DAPI (blue) for the nucleus and the corresponding colored overlays defining F-actin in the cells. (B) A linear feature detection algorithm was employed to determine total F-actin length/per cell. Each clone was plated in 8 replicates, 6 random fields were imaged and a range of 320 to 590 cells were analysed from <i>n</i> = 3 independent experiments. Scale bar, 10 μm. ****<i>P</i><0.0001.