Optic Nerve Crush Increases Stress and Correlates with an Increase in Immunoproteasome Subunits in WT Retina.

<p>(<b>A</b> and <b>B</b>) Protein was isolated from WT (●), L2 (□), and L7M1 (Δ) mouse retinas at the indicated time points after ONC. Results are the optical density of immunoproteasome subunits (<b>A</b>) LMP7 (β5i) and (<b>B</b>) LMP2 (β1i), and are the mean ± S.E. of 3–22** mice/group compared to control (day 0). (*, p≤0.05 (WT), and #, p≤0.01 (L2) by one-way ANOVA with Dunnett’s post-test compared to the ‘day 0’ values). (** All 36 day WT mouse data represents 2 mice/group). (<b>C</b>) WT retina tissue sections stained with anti-GFAP in WT retina before and after optic nerve crush (11 days). Image was taken with a 40x objective. (<b>D</b>) Protein was isolated from WT (●), L2 (■), and L7M1 (▲) retinas at the indicated time points after ONC. Results are the optical density of the GFAP protein content, and are the mean ± S.E. of 3–15 mice/group compared to control (day 0) for each mouse strain. (*, p≤0.001 (WT), #, p≤0.01 (L2) by one-way ANOVA with Dunnett’s post-test compared to the ‘Day 0’ values). (<b>E</b>) GFAP and LMP7 protein content isolated from WT (●) or L2 (□) mice are shown as a correlation. (R² = 0.2292; p≤0.01 (WT), R² = 0.1620; p≤0.01 (L2), by Pearson Correlation). (<b>F</b>) GFAP and LMP2 protein content from WT (●) or (Δ) L7M1 mice are shown as a correlation. (R² = 0.2860; p≤0.001 (WT), R² = 0.0126, p = 0.57 (L7M1), by Pearson Correlation).