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Biologically functional IgE of immunized mice was analyzed by mediator release assays.

  • Ulrike Pichler (355978)
  • Michael Hauser (207723)
  • Martin Wolf (740172)
  • Maria Livia Bernardi (171146)
  • Gabriele Gadermaier (207718)
  • Richard Weiss (740173)
  • Christof Ebner (368198)
  • Hidenori Yokoi (740174)
  • Toshiro Takai (740175)
  • Alain Didierlaurent (740176)
  • Chiara Rafaiani (171169)
  • Peter Briza (207732)
  • Adriano Mari (171186)
  • Heidrun Behrendt (271112)
  • Michael Wallner (163106)
  • Fátima Ferreira (3569831)
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Publication date
May 2015
Icon DOI
10.1371/journal.pone.0120038.g007

Abstract

<p>RBL-2H3 cells were passively sensitized with pooled immune sera (n = 5 animals/ group) withdrawn at day 49, and mediator release was triggered by addition of 0.3 μg/ml allergen. Relative β-hexosaminidase activity was calculated compared to Triton X-100 treated cells. Results are presented as mean of duplicates ± SEM. Statistical analysis was performed with One-way ANOVA and Tukey´s Multiple Comparison Test (* <i>P</i>< .05, ** <i>P</i>< .01, *** <i>P</i>< .001).</p

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