Protein profiling between metastasized tumors of control and SB-431542-treated groups.

<p>(A) A PCA plot based on the peptide profile in control and SB-431542-treated tumors using SMCA13 for the multivariate analysis and (B) corresponding loading plot. (C) OPLS-DA plot discriminates proteins from those two groups. Black dots represent the control group (n = 4) and white dots represent the SB-431542-treated group (n = 4). (D) S-plot shows the significance of protein variations between control and SB-431542-treated groups. Each black dot represents individual proteins. Black dots in the positive directions indicate the decreased proteins in SB-431542-treated tumors. The increased proteins in SB-431542-treated tumors are presented as dots in the negative direction. Vimentin (Vim), Hsp90ab1 and Eno1 were double-line-circled as the top proteins of the highest confidence and greatest contribution separation between the untreated and SB-431542-treated mice. (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126483#pone.0126483.s005" target="_blank">S1 Table</a>). A magnified picture of the significantly reduced proteins in SB-431542-treated tumors is shown (p(corr) > 0.80).