<div><p>Expression of mutant EcoRII methyltransferase protein (M.EcoRII-C186A) in <i>Escherichia coli</i> leads to tightly bound DNA-protein complexes (TBCs), located sporadically on the chromosome rather than in tandem arrays. The mechanisms behind the lethality induced by such sporadic TBCs are not well studied, nor is it clear whether very tight binding but non-covalent complexes are processed in the same way as covalent DNA-protein crosslinks (DPCs). Using 2D gel electrophoresis, we found that TBCs induced by M.EcoRII-C186A block replication forks <i>in vivo</i>. Specific bubble molecules were detected as spots on the 2D gel, only when M.EcoRII-C186A was induced, and a mutation that eliminates a specific EcoRII methylation site led to d...
SummaryDuring chromosome synthesis in Escherichia coli, replication forks are blocked by Tus bound T...
ABSTRACT We have previously identified Vibrio cholerae mutants in which the stress response to subin...
<p>A DNA-protein crosslink is a covalent bond between DNA and a protein. It is a type of DNA damage ...
Expression of mutant EcoRII methyltransferase protein (M.EcoRII-C186A) in Escherichia coli leads to ...
Methylating agents such as N-methyl-N0-nitro-N-nitrosoguanidine (MNNG) and methyl methane sulfonate ...
All organisms require mechanisms that resuscitate replication forks when they break down, reflecting...
PriA protein provides a means to load the DnaB replicative helicase at DNA replication fork and D lo...
The circular chromosome of bacteria is replicated by two replisomes assembled at the unique origin a...
All organisms require mechanisms that resuscitate replication forks when they break down, reflecting...
All organisms require mechanisms that resuscitate replication forks when they break down, reflecting...
To preserve genome integrity, all living organisms have developed strategies to respond to chromoso...
AbstractAnti-restriction and anti-modification (anti-RM) is the ability to prevent cleavage by DNA r...
<p>The bidirectional replication of a circular chromosome by many bacteria necessitates proper termi...
International audienceIntroduction DNA damage repair (DDR) is an essential process for living organi...
Diversification of the genetic code in response to selective pressures can render organisms more fit...
SummaryDuring chromosome synthesis in Escherichia coli, replication forks are blocked by Tus bound T...
ABSTRACT We have previously identified Vibrio cholerae mutants in which the stress response to subin...
<p>A DNA-protein crosslink is a covalent bond between DNA and a protein. It is a type of DNA damage ...
Expression of mutant EcoRII methyltransferase protein (M.EcoRII-C186A) in Escherichia coli leads to ...
Methylating agents such as N-methyl-N0-nitro-N-nitrosoguanidine (MNNG) and methyl methane sulfonate ...
All organisms require mechanisms that resuscitate replication forks when they break down, reflecting...
PriA protein provides a means to load the DnaB replicative helicase at DNA replication fork and D lo...
The circular chromosome of bacteria is replicated by two replisomes assembled at the unique origin a...
All organisms require mechanisms that resuscitate replication forks when they break down, reflecting...
All organisms require mechanisms that resuscitate replication forks when they break down, reflecting...
To preserve genome integrity, all living organisms have developed strategies to respond to chromoso...
AbstractAnti-restriction and anti-modification (anti-RM) is the ability to prevent cleavage by DNA r...
<p>The bidirectional replication of a circular chromosome by many bacteria necessitates proper termi...
International audienceIntroduction DNA damage repair (DDR) is an essential process for living organi...
Diversification of the genetic code in response to selective pressures can render organisms more fit...
SummaryDuring chromosome synthesis in Escherichia coli, replication forks are blocked by Tus bound T...
ABSTRACT We have previously identified Vibrio cholerae mutants in which the stress response to subin...
<p>A DNA-protein crosslink is a covalent bond between DNA and a protein. It is a type of DNA damage ...