High allelic exclusion of H and L chains in peripheral B cells of TgV_H/L3B4 mice.

<p>(A) Expression of H chains in the peripheral B cells. Cells from spleen, PEC and peripheral blood of TgV_H/L3B4 mice were stained and analyzed as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0125747#pone.0125747.g001" target="_blank">Fig 1A</a>. (B-C) Bar graph shows the absolute numbers of total, IgM^a+IgM^b- and IgM^a+IgM^b+ B cells as gated in A in the spleen (B) and PEC (C) of the indicated mice. Error bars show mean ±SD, *<i>P</i><0.05. (D) L chain expression in B cells from TgV_H/L3B4 mice. Cells were stained with anti-κ, anti-λ and anti-CD19, and were analyzed by FACS. (E) RT-PCR analysis of Rag2 mRNA expression in B cells of spleen and BM from transgenic mice and littermates. Data are representatives of five independent experiments.