Whole-transcriptome analysis of atypical and classical MBCs from parasitemic, but asymptomatic, subjects.

<p>Heat map rows represent individual genes, and columns within each MBC grouping represent distinct individuals. Representative genes are depicted based on gene ontology associations with specific functional categories. Average fold difference in expression between atMBCs and classical MBCs pairs is shown, with values in parentheses representing lower expression in atMBCs and all other values representing higher expression in atMBCs. The red and blue heat map is a graphical depiction of the significant differential regulation of each gene in nonclassical memory B cell subsets in the context of HIV infection [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref018" target="_blank">18</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref019" target="_blank">19</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref045" target="_blank">45</a>], CVID [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref022" target="_blank">22</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref023" target="_blank">23</a>], SLE [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref021" target="_blank">21</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref024" target="_blank">24</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref025" target="_blank">25</a>], HCV infection [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref026" target="_blank">26</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref046" target="_blank">46</a>], and the tonsil [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref027" target="_blank">27</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref028" target="_blank">28</a>], as well as previously reported expression in atMBCs in the context of malaria [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref011" target="_blank">11</a>,<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004894#ppat.1004894.ref031" target="_blank">31</a>]. Direction of expression change was assigned based on previously published transcriptome and protein expression profiles as described in the methods, with red representing higher expression in nonclassical subsets, blue representing lower expression, and white representing the lack of any reported change.