Induction of immune cell proliferation by NP cell seeded matrices.

<p>NP cell-seeded matrices were co-cultured with CFSE-labeled donor-type <b>(donor)</b> or allogeneic <b>(allo)</b> PBMC and incubated for 5 days. Immune cell subpopulations were identified by gating on antibody-labeled populations and the extent of proliferation was determined from plots of the CFSE signal for: all viable cells <b>(A)</b>, the T cell subsets CD3+CD8+ <b>(B)</b>, CD3+CD4+ <b>(C)</b>, activated CD25+ cells <b>(D)</b> and CD3-CD56+ NK cells <b>(E)</b>. Results are shown as mean ± SEM for n = 5 donors. A representative photograph of matrix seeded with severely degenerated NP co-cultured with allo PBMC is shown <b>(F)</b>.