Knockdown of <i>DRG-1</i> reduces cell growth and transformation potential.

<p>(A) shRNA-mediated knockdown of <i>DRG-1</i>. <i>DRG-1</i>-specific lentiviral shRNAs were constructed and transfected into <i>DRG-1</i>-expressing 293T cells. Knockdown efficiency was determined by western blot analysis. β-actin was used as a loading control. (B) Proliferative ability of <i>DRG-1</i> knockdown melanoma cells. Cell proliferation was determined by [^<b>3</b>H]thymidine incorporation in 155mel cells transfected with either control or <i>DRG-1</i>-specific shRNAs. Data are expressed as 10^<b>3</b> counts per minute (CPM). (C) Proliferative ability of <i>DRG-1</i>-overexpressing melanoma cells. Cell proliferation as assessed by [^<b>3</b>H]thymidine uptake in 155mel cells transfected with either a retrovirus vector expressing <i>DRG-1</i> or empty vector. (D) Soft agar colony formation assay was used to determine the transformation ability of 155mel cells transfected with either control or <i>DRG-1</i>-specific shRNAs.