Identification and characterization of DRG-1 peptides capable of stimulating CD4⁺ T cells.

<p>(A) Four putative DRG-1 peptides recognized by TIL155-C1 cells. (B) Identification of the DRG-1 peptide required for T cell recognition. 293DR11 cells were pulsed with four DRG-1 and irrelevant peptides and tested for T cell recognition based on IFN-γ release from TIL155-C1 cells. (C) Determination of peptide concentrations required for T cell recognition. 293IMDR11 cells were incubated with the indicated concentrations of DRG-1₂₄₈ and DRG-1₂₈₁ peptides for 90 min and washed 3 times with T-cell assay medium. T cells were added to peptide-pulsed 293IMDR11 cells overnight. IFN-γ release from T cells was determined by ELISA.