Mutant lamins mislocalize in Drosophila larval body wall muscles and have minimal dominant effects on nuclear stiffness.

<p>(A) Immunohistochemical staining of Drosophila body wall muscles from transgenic larvae expressing full length wild type (WT) and mutant Lamin C. Phalloidin staining is represented by magenta, Lamin C by green, and DAPI by blue. (B) Normalized nuclear strain values for nuclei in Drosophila larval body wall muscle. Eleven nuclei from three different larvae were analyzed per genotype. Error bars represent standard error of the mean. Increased normalized nuclear strain values correspond to decreased nuclear stiffness. The ** indicates p ≤ 0.01 compared to the value obtained for muscles expressing wild type Lamin C.