Real-time qPCR analysis of apoptosis and cell cycle-related proteins normalized against the β-actin gene.
- Hamed Karimian (533841)
- Mehran Fadaeinasab (720464)
- Soheil Zorofchian Moghadamtousi (533836)
- Maryam Hajrezaei (742977)
- Mahboubeh Razavi (742978)
- Sher Zaman Safi (742979)
- Mahmood Ameen Abdulla (112793)
- Hapipah Mohd Ali (112784)
- Mohamad Ibrahim Noordin (742980)
Publication date
May 2015
DOIAbstract
<p>Effect of polycerasoidin on mRNA expression of treated MCF7 cells. MCF7 cell were treated with IC<sub>50</sub> concentration of polycerasoidin for 12, 24 and 48 h. Quantitative analysis showed a time-dependent downregulation of Bcl-2 and upregulation of Bax, caspase 9, caspase 7, p21 and p27 in polycerasoidin-treated MCF7 cells. The data are shown as the means ± SEM. Values are statistically significant at *<i>P</i><0.05.</p
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