IL-6 secretion was not affected by alterations in F-actin or α-tubulin structures.

<p>IL-6 secretion from control and TREK-1 deficient cells was measured after exposure to cytochalasin D (Cyto D), jasplakinolide (Jasp), nocodazole (noco) with or without TNF-α for 2 (Fig 6A) or 6 hours (Fig 6B). TREK-1 overexpression had no additional effect on TNF-α-induced IL-6 secretion Fig 6C). *compared to untreated control cells; ^compared to untreated TREK-1 deficient cells; #TNF-α-stimulated control cells with and without cytochalasin D. n = 6–9.