Regulation of F-actin architecture in control, TREK-1 deficient and TREK-1 overexpressing cells.

<p>Confocal images using rhodamine-phalloidin staining (red) for F-actin filaments in untreated cells and after 6 hours of treatment with cytochalasin D (0.1 μM), jasplakinolide (0.05 μM) and TNF-α (5 ng/mL), or the combination of TNF-α+cytochalasin D or TNF-α+jasplakinolide. Inserts 1, 2 and 3 show the F-actin structures of a single control, TREK-1 shRNA or TREK-1 overexpressing cell, respectively. Nuclei are stained with DAPI (blue); images are representative of 4 separate experiments.