TNBC cell lines and basal-like breast cancer patients express high annexin A1.

<p>(A–C) Western blots showing annexin A1 protein expression relative to vinculin loading control in TNBC cell lines (A), ER⁺ cell lines (B), and ER-,Her2/neu⁺ and ER+, Her2/neu⁺ cell lines (C). (D) Proportion of cell lines in which annexin A1 protein was expressed at a level that was at least twice as high as the level in the MCF7 reference (ER⁺) cells. For this analysis, all the Her2+ lines (whether ER+/Her2+ or ER-/Her2+) were combined). (E) Box plots showing annexin A1 mRNA levels that are normalized by quantile normalization within basal, luminal, and HER2/neu+ breast cancers subtype patients. * indicates that basal subtype is statistically different than others at p<0.001. Cell line based experiments were repeated three times.