<i>Grb10</i> expression is reduced in <i>Nf1</i> mutant tumor cell lines.

<p>A. <i>Grb10</i> underexpression in tumor cell lines (SCCA is a squamous cell carcinoma cell line that arose in an irradiated <i>Nf1</i>^<i>+/-</i> mouse). A heatmap representing data from a Rfdcdfuiop\saaa vb Qiagen PI3K Array comparing expression of PI3K-pathway related transcripts among tumor cell lines and <i>Nf1</i>^<i>+/-</i> MEFs as control. <i>Grb10</i> expression is reduced in tumors compared to MEF control (yellow area at mid-column). B. Western blotting for neurofibromin protein and actin control. C. QPCR analysis of <i>Grb10</i> mRNA normalized to β-Actin shows significant decrease of Grb10 levels in tumor lines derived from <i>Nf1</i>^<i>+/-</i> mice compared to normal adult tissue controls. D. Western blotting for Grb10 in corresponding tumor cell lysates shows uniformly reduced Grb10 protein levels as compared to MEF controls.