Inhibition of mitochondrial fusion influences mitosis through cyclin B.

<p>(A) Doublet-discrimination module operating on all events to identify single cells with unreplicated and replicated DNA on the basis of pulse area and pulse width using the DNA stained by propidium iodide to generate a signal. Gate identifies single cells used for further analysis. (B) Negative control for the cyclin B staining, shows S2R⁺ cells treated with secondary antibody but not the primary anti-cyclin B. Fluorescence detected (Y-axis) is non-specificty, and is used to determine the gate for the cyclin B positive population. (C) Controls (S2R⁺ cells treated with gfp dsRNA) contain 12.2% cyclin B positive cells. (D) In S2R⁺ cells depleted of mitofusin, the population of cyclin B positive cells increases to 18.8%. (E, F) The content of cyclin B in the positive population (gates in C, D) increases from 66.3 a.u (controls, E) to 71.7 a.u. upon depletion of mitofusin (F).