shPRX3 cells are less sensitive to TS than WT MM cells.

<p>(A) PRX3 expression in cells treated with scrambled control (Sc) or PRX3 siRNA for 48 and 72 hr. (B) Cell morphology and density of cells as treated in panel A after 72 hr. (C) Cell number in HMshPRX3 cells as compared to controls over 4 days (n = 4, *** p < 0.001). (D and E) Transcript levels for PRX3 and FOXM1 in WT HM, HMshCtrl cells and HMshPRX3 cell lines (** p < 0.01, * p < 0.05, n.s. = not significant). (F) Cell lysates from HMshCtrl and HMshPRX3 cell lines incubated with 5 μM TS for 18 hr were examined for PRX3, FOXM1, and actin expression by immunoblotting after denaturing SDS-PAGE. (G) HMshCTRL and HMshPRX3 cells were treated with 5 μM TS, lysates were collected over time and examined for PRX3 by immunoblotting (ns = non-specific band). (H) Cell number in shPRX3/pZeo, shPRX3/CAT, and shPRX3/mCAT cells compared to HM controls measured over 4 days (n = 4), ** p < 0.01) (I) HM controls, shPRX3/pZeo, shPRX3/CAT, and shPRX3/mCAT cells were incubated with increasing concentrations of TS for 18 hr, and total cell mass was determined by staining with crystal violet (n = 4, ** statistically significant all groups compared to HM, p <0.01). Error bars represent SEM. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127310#pone.0127310.s003" target="_blank">S3 Fig</a>.