SmsDX inhibits the nuclear translocation of p65 induced by MCM in LNCaP, DU145 and PC-3 cells.

<p>(A, C, E)The expression of p65 in the cytoplasm was decreased after co-culture with MCM, smsDX attenuated this effect in LNCaP, DU145 and PC-3 cells. A-tubulin was chosen as the cytosolic protein loading control. (B, D, F) Co-culture with MCM increased the expression of nuclear p65, and treatment with smsDX weakened the effect of MCM. Histones were chosen as the nuclear protein loading control. The quantitation of the C-p65/ a-tubulin and N-p65/histone ratio was performed as densitometry analysis.