Transfection of synthetic miR-423-3p mimic negatively regulates Cox6a2, Ndufb7, and Ndufs5 as well as ATP level during C2C12 myotube induction.

<p>(A) Relative abundance of miRNA-423-3p by qPCR post miRNA-423-3p mimic transfection (n = 3). (B) 24 hours post-transfection, the cellular ATP level was lower (not significant) in the miR-423-3p mimic-transfected cells compared to the negative control at D0 and D4 and was significantly lower at D8. (C) Significant down-regulation of Cox6a2, Ndufb7, and Ndufs5 by qPCR 24 hours post-transfection. * and ** denotes p-values ≤ 0.05 and ≤ 0.01, respectively. All values are presented as mean ± SEM (n = 3).