The metabolic activities, protein expression ratio and mRNA expression ratio of UGT2B7 in adjacent normal and HBV-positive HCC tissues.

<p>(A) Metabolism kinetics of zidovudine in commercial, adjacent normal and HBV-positive HCC HLMs (n = 5). (B) Protein expression ratio of UGT2B7 in adjacent normal (N) and HBV-positive HCC (T) HLMs. Calnexin was applied for protein loading normalization. (C) mRNA expression ratio of UGT2B7 in adjacent normal (N) and HBV-positive HCC (T) tissues. GAPDH was used for normalization. Each data point represents the average of three determinations. Representative data from three independent experiments are shown as the mean ± S.D, *, P<0.05; **, P<0.01; ***, P<0.001. (D) The pairwise correlation of UGT2B7 between metabolic activity reduction ratio, protein expression reduction ratio and mRNA expression reduction ratio. (E) The pairwise correlation of UGT2B7 between metabolic activities, protein expression ratio and mRNA expression ratio in the adjacent normal tissues or HBV-positive HCC tissues. Bivariate correlation analysis was used to analyze the relationship of all indexes (95% confidence interval).