The concentration procedure for dengue virus (DENV) virions using sulfated sugar chain-immobilized gold nanoparticles (SGNPs).

<p>A DENV solution was diluted 1:10⁴ or 1:10⁶ in PBS. PPT, precipitate obtained from the treatment with SGNP; SUP, supernatant obtained from the treatment with SGNP; No SGNP, sample without treatment with SGNP. T_m for DENV-1 was 85 ± 0.3°C. Upper: fluorescent intensity vs. cycle number (Ct); Lower: melting curve.